Published: Journal of Chromatographic Science, Volume
37, Number 9, October 1999, pp. 383-387
An Automated Solid-Phase Microextraction– Gas Chromatography–Mass
Selective Detection Approach for the Determination of Sugar-Amino Acid Reaction
Mechanisms
W.M. Coleman, III and S.N. Lawson
Confirmation of the proposed mechanisms of Strecker aldehyde
involvement in the production of branched-chain alkyl-substituted pyrazines is
easily accomplished using an automated solid-phase microextraction–gas chromatography–mass
selective detection (AutoSPME–GC–MSD) approach. The method is relatively
elementary with minimal sample preparation. Microwave heated aqueous formulations
require only the addition of NaCl to “salt-out” the analytes prior to
analysis by AutoSPME–GC–MSD. A 100-µm polydimethylsiloxane fiber
with accompanying fiber vibration can extract ample quantities of the compounds
of interest in as little as 1 min. Such sensitivity negates the requirements for
time and reagent-consuming sample extraction via organic solvents or concentration
using heat. This approach, therefore, should find additional expanded applications
in establishing reaction pathways.
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