Published:Journal of Chromatographic Science,
ISSN 0021-9665Volume 37, Number
11, November 1999, pp. 443-447
Development
of a Capillary Electrophoresis Method for the Characterization of Collagens
in Cartilage Tissue
Michael
Chalmers, James Huckle, and Nicholas J. CottonThe
use of capillary electrophoresis (CE) for the separation of peptides specific
to type I and type II collagen is evaluated. The aim of this work is to develop
a method to characterize cartilage, cartilage repair tissue, and tissue engineered
cartilage. The analysis is dependent on the cleavage of collagen into constituent
peptides by cyanogen bromide. A number of these peptides are specific to the collagen
type. CE is evaluated for the separation of these specific peptides using uncoated
and coated capillaries over a wide range of pH and buffer concentrations. Separation
of peptides specific to type I and type II collagen is achieved using a Supelco
CElect N capillary and a 100mM phosphate buffer at pH 6. Meniscal cartilage is
characterized using this method. The proportion of type I collagen to type II
collagen corresponds well with that reported by others and indicates the potential
of this method for the characterization of cartilage.
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