Published:Journal of Chromatographic Science,
ISSN 0021-9665Volume 37, Number
11, November 1999, pp. 423-428
Retention
Study of Dichlorophenol Isomers by High-Performance Liquid Chromatography Using
a Mobile Phase Modified with b-CyclodextrinN.
Morin, E. Peyrin, S. Cornet, C. Guinchard, and Y.C. GuillaumeThe retention mechanism of a
series of 6 positional isomers of dichlorophenol in high-performance liquid chromatography
(HPLC) are investigated using b-cyclodextrin as a mobile phase additive. The values
of enthalpy and entropy of the transfer of an isomer from the mobile to the stationary
phase and the association of an isomer with b-cyclodextrin
are determined. These data demonstrate the leading role of enthalpy in relation
to entropy in the association process. Enthalpy–entropy compensation reveal
that the retention mechanism is independent of the chloro group position on the
phenol ring. The stoichiometry of the inclusion complexes between the dichlorophenol
isomers and b-cyclodextrin is determined as 1:3. The association constants of
the inclusion complexes are calculated.
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