Published: Journal of Chromatographic Science, Volume 37, Number 5, May 1999, pp. 155-158
Separation of Polyhydroxylflavonoids by Packed-Column Supercritical
Fluid Chromatography
Zhimin Liu, Suoqi Zhao, R. Wang, and Guanghua Yang
Polyhydroxylflavonoids, isorhamnetin, kampcetin, quercetin, and fisetin are efficiently separated using supercritical carbon dioxide modified by ethanol with phosphoric acid as an additive on packed-column supercritical fluid chromatography. The effect of the silica-based stationary phase, composition of mobile phase, temperature, and pressure are investigated. The addition of phosphoric acid as a polar additive improves peak shapes.
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