Published:Journal of Chromatographic Science,
ISSN 0021-9665Volume
37, Number 6, June 1999, pp. 219-226
Quantitative Applications
of Fluorescence and Ultraviolet Scanning Densitometry for Compositional Analysis
of Petroleum Products in Thin-Layer ChromatographyVicente L. Cebolla, Luis Membrado, María
P. Domingo, Patrick Henrion, Rosa Garriga, Paula González, Kimika Fakultatea,
Euskal Herriko, and Jesús Vela
Direct application of the fluorescence response enhancement (derived from
the interaction of the berberine cation with saturated hydrocarbons) to petrochemical
analysis is achieved. Hydrocarbon types are successfully determined in middle
(gasoil) and heavy (lubricants, vis-breaking fuel, heavy oil) petroleum distillates
with adequate precision and sensitivity. The novelty of this method resides
in the fact that a single fluorescence scanning is only needed for the determination
of saturates and aromatics in the same chromatogram. In spite of their chemical
inertness, saturated hydrocarbons can be quantitatively determined using thin-layer
chromatographic densitometry. The fluorescent response depends on the alkane
structure. A reliable analysis depends on the adequate choice of variables that
influence fluorescence response, precision, and sensitivity: sample load, application
volume, beam size, and impregnation conditions (berberine concentration and
impregnation time). Sensitivity of the analysis can be tailored to a certain
extent through control of the last parameter. The quantitative results agree
with those provided using other well-established techniques in the petrochemical
industry.
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