Published:Journal of Chromatographic Science,
ISSN 0021-9665Volume
37, Number 6, June 1999, pp. 199-202
Determination of Orbifloxacin
in Rabbit Plasma by High-Performance Liquid Chromatography with Fluorescence
DetectionMar’a A. Garc’a, Carlos Solans, Josˇ J. Aramayona,
Silvia Rueda, and Miguel A. Bregante
A simple and sensitive high-performance liquid chromatography method is developed
for the determination of orbifloxacin (ORB) in rabbit plasma. Sample preparations
are carried out by adding phosphate buffer (pH 7.4, 0.1M) and extracting with
trichloromethane. ORB and the internal standard, norfloxacin (NOR), are separated
on a reversed-phase column using an aqueous phosphate buffer–acetonitrile
(80:20, v/v) mobile phase. The concentrations of ORB and NOR eluting from the
column with retention times of 2.16 and 3.09 min, respectively, are monitored
by fluorescence detection at 338 (excitation) and 425 nm (emission). The method
is shown to be linear from 4 to 1500 ng/mL (regression coefficient r2 = 0.999).
The quantitation and detection limits are 4 and 9 ng/mL, respectively. Mean
recovery is determined as 92% by the analysis of plasma standards containing
150, 750, and 1500 ng/mL. Inter- and intra-assay precisions were 4 and 3%, respectively.
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