Published:Journal of Chromatographic Science,
ISSN 0021-9665Volume
37, Number 6, June 1999, pp. 191-198
Study of the Degraded Products
of N-Chloroserine in Chlorinated Waters Using High-Performance Liquid ChromatographyJ.J.
Lamelas and J. Franco, J.M. Antelo, M. Varela, and E. Falqué-López*
The most widely used method for identification of decomposed products of N-chloroamino
acids is the qualitative 2,4-dinitrophenylhydrazine method. However, this method
has serious drawbacks. For this reason, a new method based on high-perfomance
liquid chromatography that permits the identification and quantification of
the reaction products is proposed. Included in this study is the optimization
of the method (linearity, repeatability, reproducibility, and recovery) and
its application to N-chloroserine, the use of which can be easily extended to
other amino acids (the l-serine being a simple structure amino acid that has
proved to be plentiful in both drinking and residual water). To study the degradation
products of N-chloroserine, an ion-exchange capillary column and detection by
refractive index coupled with ultraviolet detection at 210 nm have been used.
The compounds determined are glycolaldehyde and b-hydroxypyruvic
acid. This identification is carried out in different mediums because the predominance
of one product or another depends on the pH value. Finally, it is shown that
the determined products are coherent with the reaction mechanism proposed for
the process.
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