Published:Journal of Chromatographic Science,
ISSN 0021-9665Volume
37, Number 7, July 1999, pp.251-254
Separation of Nucleotide Oligomers
by Unitary Anion-ExchangeC.H. Lochmüller1, Qicai Liu, Lingyan
Huang, and Ying Li
This paper is the first report on the retention behavior of synthetic oligonucleotides
and nucleotide oligomers on a continuous-bed-matrix, strong-anion-exchange column.
The separation mechanism is predominantly an anion-exchange process, but hydrophobic
interaction plays a role as well. The separation is based on the chain length
of the oligonucleotide. Both the addition of organic mobile phase modifiers
and changes in column temperature affect the retention of oligomers significantly.
A volatile buffer system (e.g., triethylamine acetate) could be employed to
purify oligonucleotides, and no desalting procedure would be required after
the column separation step. The recoveries from the separation are 70% or higher.
The maximum loading capacity of an analytical column (35 x 7-mm i.d.) was found
to be more than 366 µg.
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