Published:Journal of Chromatographic Science,
ISSN 0021-9665Volume
37, Number 7, July 1999, pp.237-239
Determination of Sterols in
Sea Urchin Gonads by High-Performance Liquid Chromatography With Ultraviolet
DetectionJ. López-Hernández*, M.J. González-Castro,
and M. Piñeiro-Sotelo
This paper is the first report on the retention behavior of synthetic oligonucleotides
and nucleotide oligomers on a continuous-A reversed-phase high-performance liquid
chromatography (HPLC) method for the determination of sterols in sea urchin
(Paracentrotus lividus) gonads is described. After saponification with KOH,
samples are extracted with hexane, evaporated to dryness, and dissolved in HPLC
eluant. Sterols were separated using a C18 column as the stationary phase, methanol–acetonitrile
(30:70, v/v) as the mobile phase, and ultraviolet detection at 205 nm. Three
sterols were identified and quantitated: desmosterol, fucosterol, and cholesterol.
Relative standard deviations (%) were 2.32 for desmosterol, 2.31 for fucosterol,
and 2.13 for cholesterol. Limits of detection were 2.31, 0.56, and 2.33 mg/mL
for desmosterol, fucosterol, and cholesterol, respectively.
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