Published:Journal of Chromatographic Science,
ISSN 0021-9665Volume 37, Number
8, August 1999, pp. 283-287
Quantitative
Determination of Indapamide in Pharmaceuticals and Urine by High-Performance
Liquid Chromatography with Amperometric Detection
M.J.
Legorburu, R.M. Alonso, R.M. Jiménez, and E. OrtizA
high-performance liquid chromatographic method with amperometric detection for
the determination of the diuretic indapamide using a µBondapak C18 column
is developed. The mobile phase consists of an acetonitrile–water mixture
(45:55, 5mM) in KH2PO4–K2HPO4 (pH 4.0). The compound is monitored at +1200
mV with an amperometric detector equipped with a glassy carbon working electrode.
A liquid–liquid or solid–liquid extraction is performed prior to chromatographic
analysis to avoid the interferences found in urine matrix. Percentages of recovery
are 88.3 ± 5.6 and 82.9 ± 7.8 for liquid–liquid and solid–liquid
extraction, respectively. The developed method has a linear concentration range
from 25 to 315 ng/mL with a reproducibility in terms of relative standard deviation
of 4% for a concentration level of 0.5 µg/mL and a quantitation limit of
1 ng/mL. The method is applied to the determination of indapamide in tablets and
urine obtained from hypertensive patients after the ingestion of Tertensif (indapamide
2.5 mg).
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