Published: Journal of Chromatographic Science, Volume 37, Number 4, April 1999, pp. 121-125.
High-Performance Liquid Chromatography of Structural Isomers
with Cyclodextrin-Poly(vinylamine)-Coated Silica Columns, Part III: Retention
Mechanism Study of Nitrophenol Derivatives
Grégorio Crini, Nadia Morin, and Michel Morcellet
The liquid chromatographic retention mechanism of nitrophenol isomers by isocratic elution is investigated over a range of column temperatures and mobile phase compositions using several b-cyclodextrin (b-CD) bonded stationary phases. The high-performance liquid chromatographic supports are based on silica beads coated with a polymer containingb-CD. Thermodynamic parameters for the solute transfer from the mobile to stationary phases are determined, and complex formation constant values between solute and b-CD are calculated. The thermodynamic data shows that the solute retention mechanism is dependent on the amount of bonded b-CD, and the complexation constant is dependent on temperature and mobile phase composition.
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