Suitability of Thin-Layer Chromatography...

Published: Journal of Chromatographic Science, Volume 36, Number 10, October 1998, pp. 487-494.

Suitability of Thin-Layer Chromatography–Flame Ionization Detection with Regard to Quantitative Characterization of Different Fossil Fuel Products. I. FID Performances and Response of Pure Compounds Related to Fossil Fuel Products
Vicente L. Cebolla, Jesús Vela, Luis Membrado, and Ana C. Ferrando

Controversy exists concerning quantitation using thin-layer chromatography–flame ionization detection (TLC–FID). Inadequate sample selections and detector designs and the non-uniformity of chromarods have been blamed for some confusing results. In this paper, performances of a modern TLC–FID system (which includes the newest detector configuration) are tested on polycyclic aromatic compound standards and related compounds as a preliminary step to evaluate its suitability for quantitative hydrocarbon group type analysis of different coal and petroleum products. Thus, evaluation of performances of the most modern FID configuration is carried out. FID linearity is evaluated as a function of sample load and scan speed for high-molecular-weight and semi-volatile standards. TLC–FID response factors for compounds of several homologous series are studied in order to differentiate effects of volatility from those exclusively due to chemical nature concerning FID response. Therefore, criteria are developed for the accurate application of TLC–FID to fossil fuel samples. Measurements of chromarod temperatures are carried out in order to evaluate whether an evaporation of compounds outside the H₂ flame might take place.

Reproduction of editorial content of this journal is prohibited without publisher’s permission.

This article is available in its entirety by fax for $4.00 per page.
Visa or MasterCard accepted.

To order electronically click here
or call: 847-647-2900 ext. 1323
or fax request to: 847-647-1155.

Please indicate JCS volume and issue along with page numbers.