Marina - A Perfusion Reversed-Phase Chromatographic Method for Ultrarapid...

Published: Journal of Chromatographic Science, Volume 36, Number 11, November 1998, pp. 527-534.

A Perfusion Reversed-Phase Chromatographic Method for Ultrarapid Determination of Soybean Proteins in Soybean Infant Formulas and Soybean Milks: Method Development and Validation
M.C. García, M. Torre1, and M.L. Marina

Perfusion reversed-phase high-performance liquid chromatography (perfusion RP-HPLC) is applied for the first time to the determination of soybean proteins in commercial samples prepared from soybean protein isolate. A linear binary gradient of acetonitrile–water–0.1% trifluoroacetic acid at a flow rate of 3 mL/min and a temperature of 60°C is optimized to analyze soybean proteins in approximately 3 min. Direct injection of samples into the chromatographic system is possible by dissolving them in water. Quantitation of soybean proteins is achieved by the proposed method using a soybean protein isolate as an external standard. The method is validated and applied to the quantitation of soybean proteins in infant formulas and powdered and liquid soybean milks.

Reproduction of editorial content of this journal is prohibited without publisher’s permission.

This article is available in its entirety by fax for $4.00 per page.
Visa or MasterCard accepted.

To order electronically click here
or call: 847-647-2900 ext. 1323
or fax request to: 847-647-1155.

Please indicate JCS volume and issue along with page numbers.