Published: Journal of Chromatographic Science, Volume 36, Number 5, May 1998, pp. 227–236.
High-Performance Liquid Chromatographic
Analysis of Amino Acid- and Peptide-Derived Chloramines
S.M. Furness-Green, T.R.
Inskeep, J.J. Starke, L. Ping, H.R. Greenleaf-Schumann, and T.E.
Goyne
An isocratic reversed-phase high-performance liquid chromatographic method is reported for the analysis of amino acid- and peptide-derived chloramines (these are important intermediates in two very different processes: destruction of microbes by the neutrophil and disinfection of water with chlorine). Specifically, results are reported for the chloramines derived from the following amino acids and dipeptides: taurine, Ala, Gly, Ser, Thr, Phe, Val, AlaGly, GlyGly, PheGly, SerGly, and ValGly. Analyses are performed on a 250 x 4.6-mm C18 column using a buffered water–acetonitrile mixture as the mobile phase. For samples containing hydrophilic chloramines, an ion-pairing agent is added to the mobile phase. Two detection methods are used: direct ultraviolet (UV) detection of the chloramine at 254 nm and indirect UV detection of I3– at 350 nm following postcolumn reaction with iodide. Unexpectedly, the decomposition of amino acid-derived chloramines is found to greatly accelerate during chromatographic elution on a reversed-phase column.
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