Published: Journal of Chromatographic Science, Volume 36, Number 1, January 1998, pp. 49-54.
Improved High-Performance Liquid
Chromatographic Assay for the Determination of Procainamide and
Its N-Acetylated
Metabolite in Plasma: Application to a Single-Dose
Pharmacokinetic Study
É. Lessard, A. Fortin, A.
Coquet, P.-M. Bélanger, B.A. Hamelin, and J. Turgeon
An improved high-performance liquid chromatographic assay for the determination of procainamide and N-acetylprocainamide (NAPA) at concentrations observed up to 32 h after a single oral dose administration of procainamide to human subjects is reported. Following liquid–liquid extraction of plasma samples, procainamide, NAPA, and the internal standard (N-propionylprocainamide) are separated on a reversed-phase C8 column with retention times of 4.0, 6.7, and 13.2 min, respectively. The ultraviolet detection limit (wavelength, 280 nm) of procainamide and NAPA is 2 ng/mL (signal-to-noise ratio, 3:1), and the quantitation limit is 4 ng/mL (signal-to-noise ratio, 5:1). Intra- and interday coefficients of variation are less than 8% in the range of 20–500 ng/mL.
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