Published: Journal of Chromatographic Science, Volume 36, Number 2, February 1998, pp. 80-84.
Determination of (+)-a-Tocopherol
in Environmental Tobacco Smoke
C.H. Risner and P.R. Nelson
A high-performance liquid chromatographic method is described for the quantitation of (+)-a-tocopherol in the particulate phase of environmental tobacco smoke (ETS) collected on a 1-µm pore size Fluoropore membrane. A methanol (MeOH) extract of the membrane, which can be used for four other ETS procedures, is analyzed for (+)-a-tocopherol on a reversed-phase column with fluorescence detection at selective wavelengths of 280 nm excitation and 330 nm emission. A mobile phase of MeOH and water is used. The method is reproducible with a relative standard deviation (%) of about 12. Recovery is 88%, and the procedure is capable of detecting greater than 0.04 µg/m3 (+)-a-tocopherol in ETS. A comparison of the ETS from five commercially available cigarettes shows similar (+)-a-tocopherol concentrations. A cigarette that primarily heats tobacco yields about 6% of that amount of (+)-a-tocopherol found in ETS from tobacco-burning cigarettes. (+)-a-Tocopherol can be used as a marker for ETS respirable suspended particles (RSP) because it is found at a consistent amount in ETS RSP of 0.29%. However, sufficient amounts of RSP would have to be generated in order to detect (+)-a-tocopherol.
Reproduction of editorial content of this journal is prohibited without publisher’s permission.
| This article is available in its entirety by fax for $4.00 per page. Visa or MasterCard accepted. To order electronically click here Please indicate JCS volume and issue along with page numbers. |
Site Map: Home
| Current Issue | Subscribe
|
| Back Issues | About
Us | Meetings | Advertising
|
| Books for Sale | For
the Author | Links |
| Supplier Info |Search
|