Separation from Related Compounds...

Published: Journal of Chromatographic Science, Volume 36, Number 8, August 1998, pp. 417-421.

Separation from Related Compounds and Assay of Calcipotriol by High-Performance Liquid Chromatography
Jesusa Joyce N. Cirunay, Yvan Vander Heyden, and Jacqueline Plaizier-Vercammen

In this paper, two methods are presented. One involves the separation of calcipotriol, a new synthetic analogue, from two related compounds, specifically cholecalciferol (Vitamin D₃) and calcitriol (1,25-dihydroxyvitamin D₃). The other involves the isolation and assay of calcipotriol from a topical ointment. The study was performed with reversed-phase high-performance liquid chromatography using an RP18 column and ultraviolet detection. Applying the method of Snyder, a mobile phase mixture containing methanol–acetonitrile–water (67:23:10, v/v) was found which achieved a total separation within 18 min. A mobile phase of methanol–water (80:20, v/v) attained a slower elution of calcipotriol. For isolation and assay of calcipotriol from an ointment (Daivonex), dissolution in chloroform gave the highest recovery (> 98%). The isolation and assay process can be performed within 2 h.

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