Published: Journal of Chromatographic Science, Volume 35, Number 9, September 1997, pp. 446–450.

Subcritical Fluid Extraction of Stevia Sweeteners from Stevia rebaudiana
J. Liu, C.P. Ong, and S.F.Y. Li

In this paper, the extraction of steviol glycosides in Stevia rebaudiana, including stevioside, rebaudioside A, rebaudioside C, and dulcoside A by subcritical fluid extraction (SubFE) is investigated. A simple, efficient SubFE method is developed. The extraction conditions (extraction phase composition, extraction time, etc.) are optimized. An extraction efficiency of more than 88% is obtained using methanol as a modifier. Determination of stevioside in the leaves of Stevia rebaudiana is performed by using the SubFE method developed and capillary electrophoresis.

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