Published: Journal of Chromatographic Science, Volume 35, Number 5, May 1997, pp. 201–205.
Reversed-Phase High-Performance Liquid
Chromatographic Separation of LY309887
(Thienyl-5,10-Dideazatetrahydrofolate) Stereoisomers Using b-Cyclodextrin
as a Mobile Phase Additive
D.W. Marks
The separation of the four stereoisomers of LY309887 (thienyl-5,10-dideazatetrahydrofolate) is studied here using commercially available high-performance liquid chromatography column technology. The selectivity needed to resolve the four stereoisomers is provided by an achiral reversed-phase column used with b-cyclodextrin as a mobile phase additive. The separation is dependent on triethylamine and b-cyclodextrin concentration in the buffer portion of the eluent, eluent strength, and buffer pH.
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