Published: Journal of Chromatographic Science, Volume 35, Number 6, June 1997, pp. 259–264.
Separation of Steroids by Micellar
Electrokinetic Capillary Chromatography with Sodium Cholate
M.-W. Hsiao and S.-T. Lin
Several steroid hormones that are routinely determined in clinical settings are resolved by micellar electrokinetic capillary chromatography (MECC) in phosphate buffer (pH = 7.4, 15 kV). These steroidal compounds are separated by MECC within a short time under physiological conditions via the use of bile salt surfactant (0.04M sodium cholate) in conjunction with an appropriate organic modifier (20% methanol). The elution patterns of seven steroid hormones are determined within 9 min with a voltage of 30 kV. The effect of varying the percentage of added methanol modifier, the applied voltage, and the temperature on the migration behavior of the steroids is examined.
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