35-7/Kumazawa.../Detection of Tricyclic Antidepressants in Whole Blood...

Published: Journal of Chromatographic Science, Volume 35, Number 7, July 1997, pp. 302–308.

Detection of Tricyclic Antidepressants in Whole Blood by Headspace Solid-Phase Microextraction and Capillary Gas Chromatography
X.-P. Lee, T. Kumazawa, K. Sato, and O. Suzuki

A simple method for the extraction of four tricyclic antidepressants from whole blood by headspace solid-phase microextraction (SPME) is presented. The whole blood samples contain four drugs (amitriptyline, chlorimipramine, imipramine, and trimipramine) and are heated at 100°C in a septum-capped vial in the presence of distilled water and NaOH solution; a polydimethylsiloxane-coated SPME fiber is exposed to the headspace of the vial to allow adsorption of the drugs before capillary gas chromatography (GC) with flame-ionization detection. The headspace SPME–GC produces intense peaks for each drug with very little background noise. Recoveries of the four drugs by the present method are 5.3–12.9%. The calibration curves for the drugs show linearity in the range of 31–1000 ng/0.5 mL. The detection limits of each drug are 16–25 ng/0.5 mL. Imipramine is detectable from rat blood 5 h after oral administration of imipramine (500 mg/kg body weight); the concentration is 1.44 ± 0.209 µg/mL.

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