35-12/Li.../Determination of Phloxine B and Uranine...

Published: Journal of Chromatographic Science, Volume 35, Number 12, December 1997, pp. 573–577.

Determination of Phloxine B and Uranine in Water by Capillary Zone Electrophoresis
Q.X. Li, J.P. Alcantara-Licudine, and L.-P. Li

Phloxine B and uranine are color additives in drugs and cosmetics as well as potential photoactive insecticides. A capillary zone electrophoretic (CZE) method is developed to determine phloxine B and uranine in water. A fused-silica capillary (67cm, 75-µm i.d.) and borate buffer are used. Migration of phloxine B and uranine increases slightly as the pH of the running buffer increases between the range of 8–9. Although there are only slight effects of ionic strength on the analyte migration in the range of 0–20mM NaCl in the running buffer, the migration of phloxine B and uranine increases as the percentage of methanol in the samples increases. Methanol shows little effect on the quantitation of phloxine B and uranine. The CZE procedure is applied to determine phloxine B and uranine fortified in tap and stream water samples. Solid-phase extraction is employed to recover the analytes spiked in the water samples. Recoveries range from 87–112% for phloxine B spiked at 10–200 ppb in the tap and stream water. Uranine recoveries are 86–91% at fortification levels of 10–50 ppb in the water samples.

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