Published: Journal of Chromatographic Science, Volume 35, Number 12, December 1997, pp. 578–583.
High-Performance Liquid Chromatographic
Determination of 2-Furaldehyde and 5-Hydroxymethyl-2-furaldehyde
in Fruit Juices
F. Lo Coco, V. Novelli, C.
Valentini, and L. Ceccon
A method for the determination of 2-furaldehyde (F) and 5-hydroxymethyl-2-furaldehyde (HMF) in fruit juices by high-performance liquid chromatography (HPLC) is described.The method is based on the formation of the 2,4-dinitrophenylhydrazones of carbonyl compounds and subsequent separation of these derivatives. Derivatization is carried out by utilizing an acidic solution of 2,4-dinitrophenylhydrazine in acetonitrile. Precipitation of the derivatives of carbonyl compounds is thus avoided, and direct injection of the sample into the HPLC system is allowed. The procedure offers a high specificity and a detection limit of the order of 10–8 mol/L. Recoveries of 95–98% are obtained from apple juice spiked at different levels with both analytes. The reproducibility (mean of six determinations) is ± 2% for F and ± 3% for HMF.
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