Published: Journal of Chromatographic Science, Volume 34, Number 11, November 1996, pp. 505–516.
Separation and Determination of a-
and b-Galactose from Agar-Type Polysaccharides by
Liquid Chromatography
M.D. Robles, F.X. Niell, and
J.M. Matés
ABSTRACT
A combination of reversed-phase liquid chromatographic technique and diode-array detection is used for quantitation of the precolumn phenylisocyanate derivatives of monosaccharides. These compounds are stable for at least two months if protected from the light and kept at room temperature. The detection limit is about 5 pmol. The method exhibits good reproducibility for both retention times and chromatographic peak areas. The method also enables a rapid and reproducible determination and separation of anomeric forms of a-and b-galactose from agar-type polysaccharides.
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