Simultaneous Determination of the Major Organic Acids...

Published: Journal of Chromatographic Science, Volume 34, Number 5, May 1996, pp. 245-253.

Simultaneous Determination of the Major Organic Acids, Sugars, Glycerol, and Ethanol by HPLC in Grape Musts and White Wines
E. Falqué López and E. Fernández Gómez

A method to simultaneously identify and quantitate the major carboxylic acids, sugars, glycerol, and ethanol in wines and grape musts is proposed. The technique involves isocratic separation, an ion-exchange column, and refractive index and UV detection (at 214 nm) without sample preparation. Statistical evaluation shows that the high-performance liquid chromatographic (HPLC) method is highly reproducible and reliable. The results of the HPLC analyses and the data obtained by standard methods are compared.

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