Orthogonal Array Design for Optimizing the Capillary Zone ...

Published: Journal of Chromatographic Science, Volume 34, Number 3, March 1996, pp. 139-145.

Orthogonal Array Design for Optimizing the Capillary Zone Electrophoretic Analysis of Heterocyclic Amines
Jun Wu, Ming Keong Wong, Hian Kee Lee, and Choon Nam Ong

Orthogonal array design (OAD) has been applied to the optimization of the capillary zone electrophoretic (CZE) analysis of heterocyclic amines (HCAs), carcinogenic and mutagenic compounds that are found in cooked foods. The factors affecting separation efficiency, such as the pH of the buffer solution, organic modifier and buffer concentrations, the applied potential, and the operating temperature, are studied in two steps under OAD. In the first experiment, each variable is tested at two levels using an OA₁₆ (215) matrix to determine the most important factors and the interactions between them. Based on the results of the first step, the second experiment is implemented according to a three-level OAD with an OA₉ (34) matrix, in which more exact values for the important variables are chosen. Finally, the determined optimum conditions for the CZE analysis of the 12 HCAs are applied and evaluated.

Reproduction of editorial content of this journal is prohibited without publisher’s permission.

This article is available in its entirety by fax for $4.00 per page.
Visa or MasterCard accepted.

To order electronically click here
or call: 847-647-2900 ext. 1323
or fax request to: 847-647-1155.

Please indicate JCS volume and issue along with page numbers.