Determination of Synthetic Estrogens in Illegal...

Published: Journal of Chromatographic Science, Volume 34, Number 7, July 1996, pp. 330-333.

Determination of Synthetic Estrogens in Illegal Veterinary Formulations by HPTLC and HPLC
Elizabeth S. Nascimento, Myriam C. Salvadori and Luciane M. Ribeiro-Neto

To determine the actual amount of diethylstilbestrol, hexestrol, and dienestrol in formulations such as pellets and oily injections that are illegally available on the Brazilian market, a simple methanol extraction is used for the analysis of the pellets and an ether extraction with Sephadex columns (for clean-up) is used for the oily injections. High-performance thin-layer chromatography is used for identification (as a qualitative and semiquantitative method), and high-performance liquid chromatography is used for quantitation. The results of the analysis show that all the formulations are not in accordance with the information listed on their labels.

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