Published: Journal of Chromatographic Science, Volume 34, Number 8, August 1996, pp. 353–357.
Determination of Melengestrol Acetate
in Supercritical Fluid–Solid Phase Extracts of Bovine Fat
Tissue by HPLC-UV and GC–MS
O.W. Parks, R.J. Shadwell,
A.R. Lightfield, and R.J. Maxwell
A method is developed for the determination of melengestrol acetate in bovine fat tissue at or less than the established tolerance level of 25 ppb. The procedure uses a combination of supercritical fluid extraction (SFE) and solid-phase extraction (SPE) techniques to produce an extract suitable for analysis with either high-performance liquid chromatography with ultraviolet detection or gas chromatography–mass spectrometry. Overall recovery of the analyte from bovine fat tissue is 99.4% with a coefficient of variation of 4.14%. The SFE–SPE procedure uses a total of 12 mL of organic solvent per fat tissue sample versus more than 1.7 L consumed in current extraction procedures.
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