Published: Journal of Chromatographic Science, Volume 34, Number 4, April 1996, pp. 174-181.
On-line HPLC–HRGC–MS for the Analysis of Natural
Complex Mixtures
Luigi Mondello, Paola Dugo, Giovanni Dugo, and Keith D. Bartle
A fully automated high-performance liquid chromatographic–high-resolution gas chromatographic–mass spectrometric system (ion-trap detection) is used for the analysis of hydrocarbon and oxygenated fractions of bitter orange, sweet orange, lemon, and mandarin leaf oils. The fractions are isolated by liquid chromatography and separated by gas chromatography. Component identification is by mass spectrometry (ITD). The qualitative and quantitative composition of the oils is discussed. Addition of or contamination by sweet orange, lemon, and mandarin leaf oils to bitter orange oil is detectable because of qualitative and quantitative differences.
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