Published: Journal of Chromatographic Science, Volume 34, Number 4, April 1996, pp. 199-201.
Separation of Neutral Lipid Classes in Green Beans by High-Performance
Liquid Chromatography
M.J. Gonzalez-Castro, J. Lopez-Hernandez, J. Simal-Lozano, M.J. Oruña-Concha,
and M.E. Vazquez-Blanco
A high-performance liquid chromatographic method that employs refractive index detection, a silica column as the stationary phase, and an isocratic mobile phase (n-hexane–2-propanol–acetic acid, 100:0.14:0.07, v/v/v) is used to determine the neutral lipids in 15 samples of whole green beans (Phaseolus vulgaris L.). Three lipid classes are identified: the most abundant class comprised nonpolar lipids (sterol esters, carotenoids, and waxes); the next comprised fatty acids; triglycerides formed the least abundant class.
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