Separation and Characterization of 25-Hydroxyvitamin D3 3-Sulfate in Human ...

Published: Journal of Chromatographic Science, Volume 33, Number 2, February 1995, pp. 82-85.

Separation and Characterization of 25-Hydroxyvitamin D₃ 3-Sulfate in Human Plasma by High-Performance Liquid Chromatography
Kazutake Shimada, Kuniko Mitamura, Miyuki Mukouyama, Tomoko Okumura, and Katsuko Sugaya

The separation and characterization of 25-hydroxyvitamin D₃ 3-sulfate in human plasma are carried out by high-performance liquid chromatography. The vitamin D sulfate fraction is obtained from a plasma specimen (three volunteers) by the combined use of a Sep-Pak C₁₈ cartridge, for solid-phase extraction, and a lipophilic gel (piperidinohydroxypropyl Sephadex LH-20), for ion-exchange chromatography. 25-Hydroxyvitamin D₃ 3-sulfate is identified in the examined three specimens in two ways: its chromatographic behavior and that of its fluorescent labeled derivative using 4-[4-(6-methoxy-2-benzoxazolyl)phenyl]-1,2,4-triazoline-3,5-dione; and data obtained from the solvolysis reaction.

Reproduction of editorial content of this journal is prohibited without publisher’s permission.

This article is available in its entirety by fax for $40.00.
Visa, MasterCard & Amex accepted.

To order electronically click here
or call: 847-647-2900 ext. 1323
or fax request to: 847-647-1155.

To order multiple copies click here.

Please indicate JCS volume and issue along with page numbers.