A New Method to Study Column Efficiency and the Separation of ...

Published: Journal of Chromatographic Science, Volume 33, Number 4, April 1995, pp. 204-210.

A New Method to Study Column Efficiency and the Separation of Seven Compounds in Reversed-Phase Chromatography
Y. Guillaume and C. Guinchard

In this work, the use of an experimental design is proposed to study the height equivalent to a theoretical plate and to separate seven compounds by high-performance liquid chromatography. The optimization of the column efficiency and the separation over the experimental region are based on a special polynomial from 13 preliminary experiments. This method connects a general simplex method to a computer. A simplex trifactor (mobile phase composition/flow rate/column temperature) optimization–liquid chromatographic (STO–LC) method has been developed in our laboratory. More than half the number of experiments required in the general simplex method can be omitted. Good agreement is obtained between predicted data and experimental results.

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